// Created by libAntimony v2.8.0 model *BIOMD0000000019() // Compartments and Species: compartment c1, c2, c3; substanceOnly species $x1 in c1, x2 in c1, x3 in c2, x4 in c2, x5 in c2; substanceOnly species x6 in c2, x7 in c2, x8 in c2, x9 in c2, x10 in c2; substanceOnly species x11 in c2, x12 in c2, x13 in c3, x14 in c2, x15 in c3; substanceOnly species x16 in c2, x17 in c2, x18 in c2, x19 in c2, x20 in c2; substanceOnly species x21 in c2, x22 in c2, x23 in c2, x24 in c2, x25 in c2; substanceOnly species x26 in c2, x27 in c2, x28 in c2, x29 in c2, x30 in c2; substanceOnly species x31 in c2, x32 in c2, x33 in c2, x34 in c2, x35 in c2; substanceOnly species x36 in c2, x37 in c2, x38 in c2, x39 in c2, x40 in c2; substanceOnly species x41 in c2, x42 in c2, x43 in c2, x44 in c2, x45 in c2; substanceOnly species x46 in c2, x47 in c2, x48 in c2, x49 in c2, x50 in c2; substanceOnly species x51 in c2, x52 in c2, x53 in c2, x54 in c2, x55 in c2; substanceOnly species x56 in c2, x57 in c2, x58 in c2, x59 in c2, x60 in c2; substanceOnly species x61 in c2, x62 in c2, x63 in c2, x64 in c2, x65 in c2; substanceOnly species x66 in c2, x67 in c2, x68 in c2, x69 in c2, x70 in c2; substanceOnly species x71 in c2, x72 in c2, x73 in c2, x74 in c2, x75 in c2; substanceOnly species x76 in c2, x77 in c2, x78 in c2, x79 in c2, x80 in c2; substanceOnly species x81 in c2, x82 in c2, x83 in c2, x84 in c2, x85 in c2; substanceOnly species x86 in c2, x87 in c2, x88 in c2, x89 in c2, x90 in c2; substanceOnly species x91 in c2, x92 in c2, x93 in c2, x94 in c2, $Raf_act in c2; substanceOnly species $Ras_GTP in c2, $MEK_PP in c2, $ERK_PP in c2, $SHC_P_t in c2; substanceOnly species $EGF_EGFR_act in c2; // Assignment Rules: Raf_act := x45 + x46 + x48 + x50 + x72 + x73 + x74 + x76; Ras_GTP := x42 + x28 + x70 + x69; MEK_PP := x51 + x77; ERK_PP := x59 + x83; SHC_P_t := x33 + x34 + x35 + x36 + x37 + x38 + x39 + x40 + x91 + x92 + x93 + x94 + x64 + x65 + x66 + x67 + x68; EGF_EGFR_act := x5 + x7 + x15 + x23 + x25 + x27 + x29 + x32 + x33 + x34 + x35 + x36 + x37 + x88 + x89 + x90 + x91 + x92 + x93 + x94 + x8 + x11 + x17 + x18 + x19 + x20 + x21 + x63 + x64 + x65 + x66 + x67 + x68; k5 := piecewise(1.55, C < 3100, 0.2, C > 100000, C*-1.350000e-005 + 1.55); C := RT/(kr1/(k1*x1) + 1); // Reactions: v1: $x1 + x2 -> x3; k1*x1*x2 - kr1*x3; v2: 2x3 -> x4; k2*x3*x3 - kr2*x4; v3: x4 -> x5; k3*x4 - kr3*x5; v4: x23 + x12 -> x7; k4*x23*x12 - kr4*x7; v5: x7 -> x18 + x9; k5*x7; v6: x2 -> x6; k6*x2 - kr6*x6; v7: x5 => x8; k7*x5; v8: x5 + x14 -> x15; k8*x5*x14 - kr8*x15; v9: x23 => x18; k7*x23; v10: x6 + x16 -> x10; k10*x6*x16 - kr10*x10; v11: 2x10 -> x11; k2*x10*x10 - kr2*x11; v12: x11 -> x8; k3*x11 - kr3*x8; v13: => x2; k13; v14: x8 + x14 -> x17; k14*x8*x14 - kr14*x17; v15: x9 => x12; k15*x9; v16: x22 + x15 -> x23; k16*x22*x15 - kr16*x23; v17: x24 + x23 -> x25; k17*x24*x23 - kr17*x25; v18: x26 + x25 -> x27; k18*x26*x25 - kr18*x27; v19: x27 -> x28 + x25; k19*x27 - kr19*x28*x25; v20: x25 + x43 -> x29; k20*x25*x43 - kr20*x29; v21: x29 -> x25 + x26; k21*x29 - kr21*x25*x26; v22: x31 + x15 -> x32; k22*x31*x15 - kr22*x32; v23: x32 -> x33; k23*x32 - kr23*x33; v24: x22 + x33 -> x34; k24*x22*x33 - kr24*x34; v25: x24 + x34 -> x35; k25*x24*x34 - kr25*x35; v26: x26 + x35 -> x36; k18*x26*x35 - kr18*x36; v27: x36 -> x35 + x28; k19*x36 - kr19*x35*x28; v28: x28 + x41 -> x42; k28*x28*x41 - kr28*x42; v29: x42 -> x43 + x45; k29*x42 - kr29*x43*x45; v30: x35 + x43 -> x37; k20*x35*x43 - kr20*x37; v31: x37 -> x35 + x26; k21*x37 - kr21*x35*x26; v32: x35 -> x15 + x38; k32*x35 - kr32*x15*x38; v33: x38 -> x40 + x30; k33*x38 - kr33*x40*x30; v34: x25 -> x15 + x30; k34*x25 - kr34*x15*x30; v35: x30 -> x24 + x22; k35*x30 - kr35*x24*x22; v36: x40 => x31; (Vm36*x40)/(Km36 + x40); v37: x33 -> x15 + x40; k37*x33 - kr37*x15*x40; v38: x22 + x40 -> x39; k24*x22*x40 - kr24*x39; v39: x34 -> x15 + x39; k37*x34 - kr37*x15*x39; v40: x24 + x39 -> x38; k40*x24*x39 - kr40*x38; v41: x30 + x33 -> x35; k41*x30*x33 - kr41*x35; v42: x44 + x45 -> x46; k42*x44*x45 - kr42*x46; v43: x46 -> x41 + x44; k43*x46; v44: x47 + x45 -> x48; k44*x47*x45 - kr44*x48; v45: x48 -> x49 + x45; k45*x48; v46: x49 + x45 -> x50; k44*x49*x45 - kr44*x50; v47: x50 -> x51 + x45; k47*x50; v48: x51 + x53 -> x52; k48*x51*x53 - kr48*x52; v49: x52 -> x49 + x53; k49*x52; v50: x53 + x49 -> x54; k50*x53*x49 - kr50*x54; v51: x54 -> x47 + x53; k49*x54; v52: x55 + x51 -> x56; k52*x55*x51 - kr52*x56; v53: x56 -> x51 + x57; k53*x56; v54: x51 + x57 -> x58; k52*x51*x57 - kr52*x58; v55: x58 -> x59 + x51; k55*x58; v56: x59 + x60 -> x61; k56*x59*x60 - kr56*x61; v57: x61 -> x57 + x60; k57*x61; v58: x60 + x57 -> x62; k58*x60*x57 - kr58*x62; v59: x62 -> x55 + x60; k59*x62; v60: x6 => x86; k60*x6; v61: x16 => x13; k61*x16; v62: x8 => x87; k60*x8; v63: x17 + x22 -> x18; k16*x17*x22 - kr16*x18; v64: x24 + x18 -> x19; k17*x24*x18 - kr17*x19; v65: x26 + x19 -> x20; k18*x26*x19 - kr18*x20; v66: x20 -> x69 + x19; k19*x20 - kr19*x69*x19; v67: x71 + x19 -> x21; k20*x71*x19 - kr20*x21; v68: x21 -> x19 + x26; k21*x21 - kr21*x19*x26; v69: x31 + x17 -> x63; k22*x31*x17 - kr22*x63; v70: x63 -> x64; k23*x63 - kr23*x64; v71: x22 + x64 -> x65; k24*x22*x64 - kr24*x65; v72: x24 + x65 -> x66; k25*x24*x65 - kr25*x66; v73: x26 + x66 -> x67; k18*x26*x66 - kr18*x67; v74: x67 -> x66 + x69; k19*x67 - kr19*x66*x69; v75: x69 + x41 -> x70; k28*x69*x41 - kr28*x70; v76: x70 -> x71 + x72; k29*x70 - kr29*x71*x72; v77: x71 + x66 -> x68; k20*x71*x66 - kr20*x68; v78: x68 -> x66 + x26; k21*x68 - kr21*x66*x26; v79: x66 -> x17 + x38; k32*x66 - kr32*x17*x38; v80: x19 -> x17 + x30; k34*x19 - kr34*x17*x30; v81: x64 -> x17 + x40; k37*x64 - kr37*x17*x40; v82: x65 -> x17 + x39; k37*x65 - kr37*x17*x39; v83: x30 + x64 -> x66; k41*x30*x64 - kr41*x66; v84: x44 + x72 -> x73; k42*x44*x72 - kr42*x73; v85: x73 -> x41 + x44; k43*x73; v86: x47 + x72 -> x74; k44*x47*x72 - kr44*x74; v87: x74 -> x75 + x72; k45*x74; v88: x72 + x75 -> x76; k44*x72*x75 - kr44*x76; v89: x76 -> x72 + x77; k47*x76; v90: x77 + x53 -> x78; k48*x77*x53 - kr48*x78; v91: x78 -> x75 + x53; k49*x78; v92: x53 + x75 -> x79; k50*x53*x75 - kr50*x79; v93: x79 -> x47 + x53; k49*x79; v94: x55 + x77 -> x80; k52*x55*x77 - kr52*x80; v95: x80 -> x81 + x77; k53*x80; v96: x77 + x81 -> x82; k52*x77*x81 - kr52*x82; v97: x82 -> x83 + x77; k55*x82; v98: x83 + x60 -> x84; k56*x83*x60 - kr56*x84; v99: x84 -> x81 + x60; k57*x84; v100: x60 + x81 -> x85; k58*x60*x81 - kr58*x85; v101: x85 -> x55 + x60; k59*x85; v102: x15 -> x17; k6*x15 - kr6*x17; v103: x32 -> x63; k6*x32 - kr6*x63; v104: x33 -> x64; k6*x33 - kr6*x64; v105: x25 -> x19; k6*x25 - kr6*x19; v106: x25 + x12 -> x88; k4*x25*x12 - kr4*x88; v107: x88 -> x9 + x19; k5*x88; v108: x27 -> x20; k6*x27 - kr6*x20; v109: x27 + x12 -> x89; k4*x27*x12 - kr4*x89; v110: x89 -> x9 + x20; k5*x89; v111: x29 -> x21; k6*x29 - kr6*x21; v112: x29 + x12 -> x90; k4*x29*x12 - kr4*x90; v113: x90 -> x9 + x21; k5*x90; v114: x34 -> x65; k6*x34 - kr6*x65; v115: x34 + x12 -> x91; k4*x34*x12 - kr4*x91; v116: x91 -> x9 + x65; k5*x91; v117: x35 -> x66; k6*x35 - kr6*x66; v118: x35 + x12 -> x92; k4*x35*x12 - kr4*x92; v119: x92 -> x9 + x66; k5*x92; v120: x36 -> x67; k6*x36 - kr6*x67; v121: x36 + x12 -> x93; k4*x36*x12 - kr4*x93; v122: x93 -> x9 + x67; k5*x93; v123: x37 -> x68; k6*x37 - kr6*x68; v124: x37 + x12 -> x94; k4*x37*x12 - kr4*x94; v125: x94 -> x68 + x9; k5*x94; // Species initializations: x1 = 4962/c1; x2 = 50000/c1; x3 = 0; x4 = 0; x5 = 0; x6 = 0; x7 = 0; x8 = 0; x9 = 0; x10 = 0; x11 = 0; x12 = 81000/c2; x13 = 0; x14 = 12000/c2; x15 = 0; x16 = 0; x17 = 0; x18 = 0; x19 = 0; x20 = 0; x21 = 0; x22 = 11000/c2; x23 = 0; x24 = 26300/c2; x25 = 0; x26 = 72000/c2; x27 = 0; x28 = 0; x29 = 0; x30 = 40000/c2; x31 = 101000/c2; x32 = 0; x33 = 0; x34 = 0; x35 = 0; x36 = 0; x37 = 0; x38 = 0; x39 = 0; x40 = 0; x41 = 40000/c2; x42 = 0; x43 = 0; x44 = 40000/c2; x45 = 0; x46 = 0; x47 = 22000000/c2; x48 = 0; x49 = 0; x50 = 0; x51 = 0; x52 = 0; x53 = 40000/c2; x54 = 0; x55 = 21000000/c2; x56 = 0; x57 = 0; x58 = 0; x59 = 0; x60 = 10000000/c2; x61 = 0; x62 = 0; x63 = 0; x64 = 0; x65 = 0; x66 = 0; x67 = 0; x68 = 0; x69 = 0; x70 = 0; x71 = 0; x72 = 0; x73 = 0; x74 = 0; x75 = 0; x76 = 0; x77 = 0; x78 = 0; x79 = 0; x80 = 0; x81 = 0; x82 = 0; x83 = 0; x84 = 0; x85 = 0; x86 = 0; x87 = 0; x88 = 0; x89 = 0; x90 = 0; x91 = 0; x92 = 0; x93 = 0; x94 = 0; // Compartment initializations: c1 = 1; c2 = 1; c3 = 4.3e-006; // Variable initializations: k1 = 0.003; k1 has pipmin; kr1 = 0.228; kr1 has pmin; kr2 = 6; kr2 has pmin; k2 = 0.001; k2 has pipmin; k3 = 60; k3 has pmin; kr3 = 0.6; kr3 has pmin; k4 = 1.038e-005; k4 has pipmin; kr4 = 0.0996; kr4 has pmin; k5 has pmin; k6 = 0.003; k6 has pmin; kr6 = 0.3; kr6 has pmin; k7 = 0.003; k7 has pmin; k8 = 0.0001; k8 has pipmin; kr8 = 12; kr8 has pmin; k10 = 3.25581; k10 has pipmin; kr10 = 0.66; kr10 has pmin; kr11 = 6; kr11 has pmin; k11 = 0.001; k11 has pipmin; kr12 = 0.6; kr12 has pmin; k12 = 60; k12 has pmin; k13 = 130.2; k13 has ipmin; k14 = 0.0001; k14 has pipmin; kr14 = 12; kr14 has pmin; k15 = 600000; k15 has pmin; kr16 = 16.5; kr16 has pmin; k16 = 0.001; k16 has pipmin; kr17 = 3.6; kr17 has pmin; k17 = 0.001; k17 has pipmin; kr18 = 78; kr18 has pmin; k18 = 0.0015; k18 has pipmin; kr19 = 1e-005; kr19 has pipmin; k19 = 30; k19 has pmin; kr20 = 24; kr20 has pmin; k20 = 0.00021; k20 has pipmin; k21 = 1.38; k21 has pmin; kr21 = 2.2e-005; kr21 has pipmin; k22 = 0.0021; k22 has pipmin; kr22 = 6; kr22 has pmin; k23 = 360; k23 has pmin; kr23 = 36; kr23 has pmin; k24 = 0.001; k24 has pipmin; kr24 = 33; kr24 has pmin; kr25 = 1.284; kr25 has pmin; k25 = 0.001; k25 has pipmin; k28 = 0.0001; k28 has pipmin; kr28 = 0.318; kr28 has pmin; k29 = 60; k29 has pmin; kr29 = 7e-005; kr29 has pipmin; kr32 = 2.4e-005; kr32 has pipmin; k32 = 6; k32 has pmin; k33 = 12; k33 has pmin; kr33 = 0.0021; kr33 has pipmin; k34 = 1.8; k34 has pmin; kr34 = 0.00045; kr34 has pipmin; k35 = 0.09; k35 has pmin; kr35 = 0.00045; kr35 has pipmin; Vm36 = 61200; Vm36 has ipmin; Km36 = 200000000000000; Km36 has substance; k37 = 18; k37 has pmin; kr37 = 9e-005; kr37 has pipmin; k40 = 0.003; k40 has pipmin; kr40 = 3.84; kr40 has pmin; kr41 = 2.574; kr41 has pmin; k41 = 0.003; k41 has pipmin; k42 = 0.0071; k42 has pipmin; kr42 = 12; kr42 has pmin; k43 = 60; k43 has pmin; kr44 = 1.0998; kr44 has pmin; k44 = 0.00111; k44 has pipmin; k45 = 210; k45 has pmin; k47 = 174; k47 has pmin; kr48 = 48; kr48 has pmin; k48 = 0.00143; k48 has pipmin; k49 = 3.48; k49 has pmin; kr50 = 30; kr50 has pmin; k50 = 2.5e-005; k50 has pipmin; k52 = 0.00534; k52 has pipmin; kr52 = 1.98; kr52 has pmin; k53 = 960; k53 has pmin; k55 = 342; k55 has pmin; kr56 = 36; kr56 has pmin; k56 = 0.00145; k56 has pipmin; k57 = 16.2; k57 has pmin; kr58 = 30; kr58 has pmin; k58 = 0.0005; k58 has pipmin; k59 = 18; k59 has pmin; k60 = 0.04002; k60 has pmin; k61 = 0.01002; k61 has pmin; RT = 50000; RT has substance; // Other declarations: var k5, C; const c1, c2, c3, k1, kr1, kr2, k2, k3, kr3, k4, kr4, k6, kr6, k7, k8, kr8; const k10, kr10, kr11, k11, kr12, k12, k13, k14, kr14, k15, kr16, k16, kr17; const k17, kr18, k18, kr19, k19, kr20, k20, k21, kr21, k22, kr22, k23, kr23; const k24, kr24, kr25, k25, k28, kr28, k29, kr29, kr32, k32, k33, kr33; const k34, kr34, k35, kr35, Vm36, Km36, k37, kr37, k40, kr40, kr41, k41; const k42, kr42, k43, kr44, k44, k45, k47, kr48, k48, k49, kr50, k50, k52; const kr52, k53, k55, kr56, k56, k57, kr58, k58, k59, k60, k61, RT; // Unit definitions: unit substance = item; unit time_unit = 60 second; unit volume = 1e-12 litre; unit pmin = 1 / 60 second; unit ipmin = item / 60 second; unit pipmin = 1 / (60 second * item); unit piplpmin = 1e-12 litre / (item * 60 second); // Display Names: substance is "items"; time_unit is "min"; volume is "pl"; pmin is "permin"; ipmin is "itemspermin"; pipmin is "peritempermin"; piplpmin is "plperitempermin"; c1 is "extracellular volume"; c2 is "cytoplasm"; c3 is "endosomal volume"; x1 is "EGF"; x2 is "EGFR"; x3 is "EGF-EGFR"; x4 is "EGF-EGFR^2"; x5 is "EGF-EGFR*^2"; x6 is "EGFRi"; x7 is "EGF-EGFR*^2-GAP-Grb2-Prot"; x8 is "EGF-EGFRi*^2"; x9 is "Proti"; x10 is "EGF-EGFRi"; x11 is "EGF-EGFRi^2"; x12 is "Prot"; x13 is "EGFideg"; x14 is "GAP"; x15 is "EGF-EGFR*^2-GAP"; x16 is "EGFi"; x17 is "EGF-EGFRi*^2-GAP"; x18 is "EGF-EGFRi*^2-GAP-Grb2"; x19 is "EGF-EGFRi*^2-GAP-Grb2-Sos"; x20 is "EGF-EGFRi*^2-GAP-Grb2-Sos-Ras-GDP"; x21 is "EGF-EGFRi*^2-GAP-Grb2-Sos-Ras-GTP"; x22 is "Grb2"; x23 is "EGF-EGFR*^2-GAP-Grb2"; x24 is "Sos"; x25 is "EGF-EGFR*^2-GAP-Grb2-Sos"; x26 is "Ras-GDP"; x27 is "EGF-EGFR*^2-GAP-Grb2-Sos-Ras-GDP"; x28 is "Ras-GTP"; x29 is "EGF-EGFR*^2-GAP-Grb2-Sos-Ras-GTP"; x30 is "Grb2-Sos"; x31 is "Shc"; x32 is "EGF-EGFR*^2-GAP-Shc"; x33 is "EGF-EGFR*^2-GAP-Shc*"; x34 is "EGF-EGFR*^2-GAP-Shc*-Grb2"; x35 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Sos"; x36 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Sos-Ras-GDP"; x37 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Sos-Ras-GTP"; x38 is "Shc*-Grb2-Sos"; x39 is "Shc*-Grb2"; x40 is "Shc*"; x41 is "Raf"; x42 is "Raf-Ras-GTP"; x43 is "Ras-GTP*"; x44 is "Phosphotase1"; x45 is "Raf*"; x46 is "Raf*-P'ase"; x47 is "MEK"; x48 is "MEK-Raf*"; x49 is "MEK-P"; x50 is "MEK-P-Raf*"; x51 is "MEK-PP"; x52 is "MEK-PP-P'ase2"; x53 is "Phosphatase2"; x54 is "MEK-P-P'ase2"; x55 is "ERK"; x56 is "ERK-MEK-PP"; x57 is "ERK-P"; x58 is "ERK-P-MEK-PP"; x59 is "ERK-PP"; x60 is "Phosphotase3"; x61 is "ERK-PP-P'ase3"; x62 is "ERK-P-P'ase3"; x63 is "EGF-EGFRi*^2-GAP-Shc"; x64 is "EGF-EGFRi*^2-GAP-Shc*"; x65 is "EGF-EGFRi*^2-GAP-Shc*-Grb2"; x66 is "EGF-EGFRi*^2-GAP-Shc*-Grb2-Sos"; x67 is "EGF-EGFRi*^2-GAP-Shc*-Grb2-Sos-Ras-GDP"; x68 is "EGF-EGFRi*^2-GAP-Shc*-Grb2-Sos-Ras-GTP"; x69 is "Rasi-GTP"; x70 is "Rafi-Rasi-GTP"; x71 is "Rasi-GTP*"; x72 is "Rafi*"; x73 is "Rafi*-P'ase"; x74 is "MEKi-Rafi*"; x75 is "MEKi-P"; x76 is "MEKi-P-Rafi*"; x77 is "MEKi-PP"; x78 is "MEKi-PP-P'ase2i"; x79 is "MEKi-P-P'ase2i"; x80 is "ERKi-MEKi-PP"; x81 is "ERKi-P"; x82 is "ERKi-P-MEKi-PP"; x83 is "ERKi-PP"; x84 is "ERKi-PP-P'ase3i"; x85 is "ERKi-P-P'ase3i"; x86 is "EGFRideg"; x87 is "EGF-EGFRi*^2deg"; x88 is "EGF-EGFR*^2-GAP-Grb2-Sos-Prot"; x89 is "EGF-EGFR*^2-GAP-Grb2-Sos-Ras-GDP-Prot"; x90 is "EGF-EGFR*^2-GAP-Grb2-Sos-Ras-GTP-Prot"; x91 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Prot"; x92 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Sos-Prot"; x93 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Sos-Ras-GDP-Prot"; x94 is "EGF-EGFR*^2-GAP-Shc*-Grb2-Sos-Ras-GTP-Prot"; Raf_act is "t_Raf*"; Ras_GTP is "t_Ras_GTP"; MEK_PP is "t_MEK_PP"; ERK_PP is "t_ERK_PP"; SHC_P_t is "t_SHC_P_t"; EGF_EGFR_act is "t_EGF_EGFR*"; C is "C_internalization"; RT is "total_Receptors"; end