// Created by libAntimony v2.8.0 model *Pritchard2002_glycolysis() // Compartments and Species: compartment cell, ext_; species $GLCo in ext_, GLCi in cell, ATP in cell, G6P in cell, ADP in cell; species F6P in cell, F16bP in cell, AMP in cell, $F26bP in cell, DHAP in cell; species GAP in cell, NAD in cell, BPG in cell, NADH in cell, P3G in cell; species P2G in cell, PEP in cell, PYR in cell, AcAld in cell, $CO2 in cell; species $EtOH in cell, $Glycerol in cell, $Glycogen in cell, $Trehalose in cell; species $Succinate in cell; // Reactions: HXT: $GLCo -> GLCi; (HXT_Vmax_1*(GLCo - GLCi))/HXT_Kglc_1/(1 + (GLCo + GLCi)/HXT_Kglc_1 + (HXT_Ki_1*GLCo*GLCi)/HXT_Kglc_1^2); HK: GLCi + ATP -> G6P + ADP; (cell*HK_Vmax_2*((GLCi*ATP)/(HK_Kglc_2*HK_Katp_2) - (G6P*ADP)/(HK_Kglc_2*HK_Katp_2*HK_Keq_2)))/((1 + GLCi/HK_Kglc_2 + G6P/HK_Kg6p_2)*(1 + ATP/HK_Katp_2 + ADP/HK_Kadp_2)); PGI: G6P -> F6P; (cell*PGI_Vmax_3*(G6P/PGI_Kg6p_3 - F6P/(PGI_Kg6p_3*PGI_Keq_3)))/(1 + G6P/PGI_Kg6p_3 + F6P/PGI_Kf6p_3); PFK: ATP + F6P => ADP + F16bP; cell*PFK_Vmax_4*((PFK_gR_4*(F6P/PFK_Kf6p_4)*(ATP/PFK_Katp_4)*(1 + F6P/PFK_Kf6p_4 + ATP/PFK_Katp_4 + ((PFK_gR_4*F6P)/PFK_Kf6p_4*ATP)/PFK_Katp_4))/((1 + F6P/PFK_Kf6p_4 + ATP/PFK_Katp_4 + ((PFK_gR_4*F6P)/PFK_Kf6p_4*ATP)/PFK_Katp_4)^2 + PFK_L0_4*((1 + (PFK_Ciatp_4*ATP)/PFK_Kiatp_4)/(1 + ATP/PFK_Kiatp_4))^2*((1 + (PFK_Camp_4*AMP)/PFK_Kamp_4)/(1 + AMP/PFK_Kamp_4))^2*((1 + (PFK_Cf26_4*F26bP)/PFK_Kf26_4 + (PFK_Cf16_4*F16bP)/PFK_Kf16_4)/(1 + F26bP/PFK_Kf26_4 + F16bP/PFK_Kf16_4))^2*(1 + (PFK_Catp_4*ATP)/PFK_Katp_4)^2)); ALD: F16bP -> DHAP + GAP; (cell*ALD_Vmax_5*(F16bP/ALD_Kf16bp_5 - (DHAP*GAP)/(ALD_Kf16bp_5*ALD_Keq_5)))/(1 + F16bP/ALD_Kf16bp_5 + DHAP/ALD_Kdhap_5 + GAP/ALD_Kgap_5 + (F16bP*GAP)/(ALD_Kf16bp_5*ALD_Kigap_5) + (DHAP*GAP)/(ALD_Kdhap_5*ALD_Kgap_5)); TPI: DHAP -> GAP; cell*(TPI_k1_6*DHAP - TPI_k2_6*GAP); GAPDH: GAP + NAD -> BPG + NADH; (cell*GAPDH_C_7*((GAPDH_Vmaxf_7*GAP*NAD)/(GAPDH_Kgap_7*GAPDH_Knad_7) - (GAPDH_Vmaxr_7*BPG*NADH)/(GAPDH_Kbpg_7*GAPDH_Knadh_7)))/((1 + GAP/GAPDH_Kgap_7 + BPG/GAPDH_Kbpg_7)*(1 + NAD/GAPDH_Knad_7 + NADH/GAPDH_Knadh_7)); PGK: ADP + BPG -> ATP + P3G; (cell*PGK_Vmax_8*((PGK_Keq_8*BPG*ADP - P3G*ATP)/(PGK_Kp3g_8*PGK_Katp_8)))/((1 + BPG/PGK_Kbpg_8 + P3G/PGK_Kp3g_8)*(1 + ADP/PGK_Kadp_8 + ATP/PGK_Katp_8)); PGM: P3G -> P2G; (cell*PGM_Vmax_9*(P3G/PGM_Kp3g_9 - P2G/(PGM_Kp3g_9*PGM_Keq_9)))/(1 + P3G/PGM_Kp3g_9 + P2G/PGM_Kp2g_9); ENO: P2G -> PEP; (cell*ENO_Vmax_10*(P2G/ENO_Kp2g_10 - PEP/(ENO_Kp2g_10*ENO_Keq_10)))/(1 + P2G/ENO_Kp2g_10 + PEP/ENO_Kpep_10); PYK: ADP + PEP -> ATP + PYR; (cell*PYK_Vmax_11*((PEP*ADP)/(PYK_Kpep_11*PYK_Kadp_11) - (PYR*ATP)/(PYK_Kpep_11*PYK_Kadp_11*PYK_Keq_11)))/((1 + PEP/PYK_Kpep_11 + PYR/PYK_Kpyr_11)*(1 + ADP/PYK_Kadp_11 + ATP/PYK_Katp_11)); PDC: PYR => AcAld + $CO2; (cell*PDC_Vmax_12*(PYR/PDC_Kpyr_12)^PDC_nH_12)/(1 + (PYR/PDC_Kpyr_12)^PDC_nH_12); ADH: NAD + $EtOH -> NADH + AcAld; (cell*ADH_Vmax_13*((EtOH*NAD)/(ADH_Ketoh_13*ADH_Kinad_13) - (AcAld*NADH)/(ADH_Ketoh_13*ADH_Kinad_13*ADH_Keq_13)))/(1 + NAD/ADH_Kinad_13 + (EtOH*ADH_Knad_13)/(ADH_Kinad_13*ADH_Ketoh_13) + (AcAld*ADH_Knadh_13)/(ADH_Kinadh_13*ADH_Kacald_13) + NADH/ADH_Kinadh_13 + (EtOH*NAD)/(ADH_Kinad_13*ADH_Ketoh_13) + (NAD*AcAld*ADH_Knadh_13)/(ADH_Kinad_13*ADH_Kinadh_13*ADH_Kacald_13) + (EtOH*NADH*ADH_Knad_13)/(ADH_Kinad_13*ADH_Kinadh_13*ADH_Ketoh_13) + (AcAld*NADH)/(ADH_Kacald_13*ADH_Kinadh_13) + (EtOH*NAD*AcAld)/(ADH_Kinad_13*ADH_Kiacald_13*ADH_Ketoh_13) + (EtOH*AcAld*NADH)/(ADH_Kietoh_13*ADH_Kinadh_13*ADH_Kacald_13)); ATPase: ATP => ADP; cell*ATPase_Katpase_14*ATP; AK: 2ADP -> ATP + AMP; cell*(AK_k1_15*ADP*ADP - AK_k2_15*ATP*AMP); G3PDH: DHAP + NADH => NAD + $Glycerol; (cell*G3PDH_Vmax_16*(DHAP/G3PDH_Kdhap_16*(NADH/G3PDH_Knadh_16) - Glycerol/G3PDH_Kdhap_16*(NAD/G3PDH_Knadh_16)*(1/G3PDH_Keq_16)))/((1 + DHAP/G3PDH_Kdhap_16 + Glycerol/G3PDH_Kglycerol_16)*(1 + NADH/G3PDH_Knadh_16 + NAD/G3PDH_Knad_16)); Glycogen_Branch: ATP + G6P => ADP + $Glycogen; cell*Glycogen_Branch_KGLYCOGEN_17; Trehalose_Branch: ATP + 2G6P => ADP + $Trehalose; cell*Trehalose_Branch_Ktrehalose_18; Succinate_Branch: 3NAD + 2AcAld => 3NADH + $Succinate; cell*Succinate_Branch_k_19*AcAld; // Species initializations: GLCo = 2; GLCi = 0.097652231064563; ATP = 2.52512746499271; G6P = 2.67504014044787; ADP = 1.28198768168719; F6P = 0.624976405532373; F16bP = 6.22132076069411; AMP = 0.292884853320091; F26bP = 0.02; DHAP = 1.00415254899644; GAP = 0.0451809175780963; NAD = 1.50329030201531; BPG = 0.000736873499865602; NADH = 0.0867096979846952; P3G = 0.885688538360659; P2G = 0.127695817386632; PEP = 0.0632352144936527; PYR = 1.81531251192736; AcAld = 0.178140579850657; CO2 = 1; EtOH = 50; Glycerol = 0.15; Glycogen = 0; Trehalose = 0; Succinate = 0; // Compartment initializations: cell = 1; ext_ = 1; // Variable initializations: HXT_Vmax_1 = 97.24; HXT_Kglc_1 = 1.1918; HXT_Ki_1 = 0.91; HK_Vmax_2 = 236.7; HK_Kglc_2 = 0.08; HK_Katp_2 = 0.15; HK_Keq_2 = 2000; HK_Kg6p_2 = 30; HK_Kadp_2 = 0.23; PGI_Vmax_3 = 1056; PGI_Kg6p_3 = 1.4; PGI_Keq_3 = 0.29; PGI_Kf6p_3 = 0.3; PFK_Vmax_4 = 110; PFK_gR_4 = 5.12; PFK_Kf6p_4 = 0.1; PFK_Katp_4 = 0.71; PFK_L0_4 = 0.66; PFK_Ciatp_4 = 100; PFK_Kiatp_4 = 0.65; PFK_Camp_4 = 0.0845; PFK_Kamp_4 = 0.0995; PFK_Cf26_4 = 0.0174; PFK_Kf26_4 = 0.000682; PFK_Cf16_4 = 0.397; PFK_Kf16_4 = 0.111; PFK_Catp_4 = 3; ALD_Vmax_5 = 94.69; ALD_Kf16bp_5 = 0.3; ALD_Keq_5 = 0.069; ALD_Kdhap_5 = 2; ALD_Kgap_5 = 2.4; ALD_Kigap_5 = 10; TPI_k1_6 = 450000; TPI_k2_6 = 10000000; GAPDH_C_7 = 1; GAPDH_Vmaxf_7 = 1152; GAPDH_Kgap_7 = 0.21; GAPDH_Knad_7 = 0.09; GAPDH_Vmaxr_7 = 6719; GAPDH_Kbpg_7 = 0.0098; GAPDH_Knadh_7 = 0.06; PGK_Vmax_8 = 1288; PGK_Keq_8 = 3200; PGK_Kp3g_8 = 0.53; PGK_Katp_8 = 0.3; PGK_Kbpg_8 = 0.003; PGK_Kadp_8 = 0.2; PGM_Vmax_9 = 2585; PGM_Kp3g_9 = 1.2; PGM_Keq_9 = 0.19; PGM_Kp2g_9 = 0.08; ENO_Vmax_10 = 201.6; ENO_Kp2g_10 = 0.04; ENO_Keq_10 = 6.7; ENO_Kpep_10 = 0.5; PYK_Vmax_11 = 1000; PYK_Kpep_11 = 0.14; PYK_Kadp_11 = 0.53; PYK_Keq_11 = 6500; PYK_Kpyr_11 = 21; PYK_Katp_11 = 1.5; PDC_Vmax_12 = 857.8; PDC_Kpyr_12 = 4.33; PDC_nH_12 = 1.9; ADH_Vmax_13 = 209.5; ADH_Ketoh_13 = 17; ADH_Kinad_13 = 0.92; ADH_Keq_13 = 6.9e-005; ADH_Knad_13 = 0.17; ADH_Knadh_13 = 0.11; ADH_Kinadh_13 = 0.031; ADH_Kacald_13 = 1.11; ADH_Kiacald_13 = 1.1; ADH_Kietoh_13 = 90; ATPase_Katpase_14 = 39.5; AK_k1_15 = 45; AK_k2_15 = 100; G3PDH_Vmax_16 = 47.11; G3PDH_Kdhap_16 = 0.4; G3PDH_Knadh_16 = 0.023; G3PDH_Keq_16 = 4300; G3PDH_Kglycerol_16 = 1; G3PDH_Knad_16 = 0.93; Glycogen_Branch_KGLYCOGEN_17 = 6; Trehalose_Branch_Ktrehalose_18 = 2.4; Succinate_Branch_k_19 = 21.4; // Other declarations: const cell, ext_; // Unit definitions: unit substance = 1e-3 mole; unit time_unit = 60 second; // Display Names: substance is "mmole"; time_unit is "minute"; cell is "cytosol"; ext_ is "exterior"; GLCo is "Glc(ext)"; GLCi is "Glc(int)"; G6P is "Glu6P"; F6P is "Fru6-P"; F16bP is "Fru1,6-P2"; F26bP is "Fru2,6-P2"; DHAP is "glycerone phosphate"; GAP is "Gra3P"; BPG is "Gri2,3P2"; P3G is "Gri3P"; P2G is "Gri2P"; PEP is "phosphoenolpyruvate"; PYR is "pyruvate"; AcAld is "acetaldehyde"; EtOH is "ethanol"; Glycerol is "glycerol"; Glycogen is "glycogen"; Trehalose is "trehalose"; Succinate is "succinate"; HXT is "glucose transport"; HK is "hexokinase"; PGI is "phosphoglucose isomerase"; PFK is "phosphofructosekinase"; ALD is "fructosebisphosphate aldolase"; TPI is "triosephosphate isomerase"; GAPDH is "glyceraldehyde phosphate dehydrogenase"; PGK is "3-phosphoglycerate kinase"; PGM is "phosphoglyceromutase"; ENO is "enolase"; PYK is "pyruvate kinase"; PDC is "pyruvate decarboxylase"; ADH is "alcohol dehydrogenase"; G3PDH is "glycerol-3-phosphate dehydrogenase"; end