// Created by libAntimony v2.8.0 model *BIOMD0000000012() // Compartments and Species: compartment cell; substanceOnly species PX in cell, PY in cell, PZ in cell, X in cell, Y in cell; substanceOnly species Z in cell; // Assignment Rules: beta := tau_mRNA/tau_prot; alpha0 := (a0_tr*eff*tau_prot)/(ln(2)*KM); a0_tr := ps_0*60; alpha := (a_tr*eff*tau_prot)/(ln(2)*KM); a_tr := (ps_a - ps_0)*60; t_ave := tau_mRNA/ln(2); kd_mRNA := ln(2)/tau_mRNA; kd_prot := ln(2)/tau_prot; k_tl := eff/t_ave; // Reactions: Reaction1: X => ; kd_mRNA*X; Reaction2: Y => ; kd_mRNA*Y; Reaction3: Z => ; kd_mRNA*Z; Reaction4: => PX; k_tl*X; Reaction5: => PY; k_tl*Y; Reaction6: => PZ; k_tl*Z; Reaction7: PX => ; kd_prot*PX; Reaction8: PY => ; kd_prot*PY; Reaction9: PZ => ; kd_prot*PZ; Reaction10: => X; a0_tr + (a_tr*KM^n)/(KM^n + PZ^n); Reaction11: => Y; a0_tr + (a_tr*KM^n)/(KM^n + PX^n); Reaction12: => Z; a0_tr + (a_tr*KM^n)/(KM^n + PY^n); // Species initializations: PX = 0; PY = 0; PZ = 0; X = 0; Y = 20/cell; Z = 0; // Compartment initializations: cell = 1; // Variable initializations: tau_mRNA = 2; tau_prot = 10; eff = 20; KM = 40; n = 2; ps_a = 0.5; ps_0 = 0.0005; // Other declarations: var beta, alpha0, a0_tr, alpha, a_tr, t_ave, kd_mRNA, kd_prot, k_tl; const cell, tau_mRNA, tau_prot, eff, KM, n, ps_a, ps_0; // Unit definitions: unit volume = 1e-15 litre; unit substance = item; unit time_unit = 60 second; // Display Names: volume is "cubic microns"; substance is "item"; time_unit is "minute"; PX is "LacI protein"; PY is "TetR protein"; PZ is "cI protein"; X is "LacI mRNA"; Y is "TetR mRNA"; Z is "cI mRNA"; tau_mRNA is "mRNA half life"; tau_prot is "protein half life"; eff is "translation efficiency"; t_ave is "average mRNA life time"; ps_a is "tps_active"; ps_0 is "tps_repr"; Reaction1 is "degradation of LacI transcripts"; Reaction2 is "degradation of TetR transcripts"; Reaction3 is "degradation of CI transcripts"; Reaction4 is "translation of LacI"; Reaction5 is "translation of TetR"; Reaction6 is "translation of CI"; Reaction7 is "degradation of LacI"; Reaction8 is "degradation of TetR"; Reaction9 is "degradation of CI"; Reaction10 is "transcription of LacI"; Reaction11 is "transcription of TetR"; Reaction12 is "transcription of CI"; end